Lab 12 Write Up

Lab 12 Write Up - Drew Rasmussen Penny Louka Biology 191L...

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Drew Rasmussen Penny Louka Biology 191L December 4, 2010 Lab 12: Genetic Engineering and Bacterial Transformation: Lab Write Up 1. Transformed bacteria was produced in the treatment dish containing both ampicillin (in the agar substrate), and the plasmid introduced to the bacteria. 2. Dish Comparison and discussion: a. Dish 1 contained no ampicillin or plasmid, and bacteria colonies were able to grow. Since there was no ampicillin there was also no need to contain an antibiotic resistant gene, so the bacteria were able to colonize the agar. b. Dish 2 contained ampicillin and bacteria with no plasmid introduced. No growth was observed in this dish because the ampicillin killed the bacteria without the presence of the antibiotic resistant gene. c. Dish 3 contained no ampicillin, but had the plasmid. Growth was observed here even though adding the plasmid was used only to serve as a control for dish 4. Growth occurred because there were no traces of antibiotics, and even if there
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This note was uploaded on 01/18/2012 for the course BIOL 192L taught by Professor Merlinwhite during the Spring '11 term at Boise State.

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Lab 12 Write Up - Drew Rasmussen Penny Louka Biology 191L...

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