Lab 15 Pre Write Up

Lab 15 Pre Write Up - Drew Rasmussen Penny Louka Biology...

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Drew Rasmussen Penny Louka Biology 191L December 7, 2010 Lab 15: DNA Fingerprinting and Molecular Systematics: Lab Pre Write Up 1. Yes, this is very possible because all of the children have 1 allele from the mother, and one from the father. 2. PCR needs to be used because it amplifies DNA from a crime scene which makes it easier to test the STR regions using gel electrophoresis to see if there is a match between the crime scene DNA and the suspect. 3. The three steps of PCR are denaturation, annealing and extension. During denaturation the DNA is “denatured” making it single stranded. Annealing is where all the ionic bonds going on within the DNA begin to become stronger, and eventually they do not break apart from Brownian motion. Forward and reverse primers are now present, and begin to stick together during the annealing process. During the extension process bases are coupled, from the 3’ to 5’ end. This extends the DNA.
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This note was uploaded on 01/18/2012 for the course BIOL 192L taught by Professor Merlinwhite during the Spring '11 term at Boise State.

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Lab 15 Pre Write Up - Drew Rasmussen Penny Louka Biology...

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