chapter 15 review

chapter 15 review - Lecture 25 Chapter 15: Genetic...

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Lecture 25 – Chapter 15: Genetic Engineering I. DNA Cloning A. Restriction Enzymes 1. enzymes from bacteria that cut DNA molecules only in specific places 2. many cut palindromic sequences, in which the base sequence of one strand reads the same as its complement when both are read in the 5’→3’ direction 3. Sticky ends a. portion of DNA segment that can pair with singlestranded ends of other DNA molecules that were digested by the same enzyme b. DNA molecules with complementary sticky ends associate by hydrogen bonds recombinant DNA B. Vectors 1. carrier such as a bacteriophage or plasmid capable of transporting the DNA fragment into a cell 2. researchers introduce plasmids into bacterial cell by transformation, the uptake of foreign DNA by cells 3. in host cell, recombinant DNA is copied and turned on to produce gene product C. Producing Genomic Library Genome – total DNA in a cell Genomic DNA Library – collection of DNA fragments that represent all DNA in genome Chromosome Library – contains all DNA fragments in specific chromosome 1. Restriction enzyme cuts DNA, generating population of DNA fragments with
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chapter 15 review - Lecture 25 Chapter 15: Genetic...

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