Genetics Final 2010 pg 9

Genetics Final 2010 pg 9 - strand(adds new dNTP’s in the...

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ANSWERS: 1. C 2. E 3. E 4. B 5. A 11. A 12. C 13. B 14. E 19. C 20. A 21. A 22. A 23. DNA replication in E. coli always starts at Ori.C and moves in both directions on each strand until it reaches the terminus. First DNA-A binds to OriC and begins the unwinding process while topoisomerases relax the coils further away to augment the unwinding. Helicase and associated enzymes break the hydrogen bonds between bases to separate the two strands of DNA. Single-Stranded binding proteins attach to the freshly denatured DNA strands to keep them from re-annealing. Primase adds small RNA primers to the DNA so that the DNA polymerase III can bind to the double-stranded portion and create the new DNA
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Unformatted text preview: strand (adds new dNTP’s) in the 5’ 3’ direction. On the leading strand this is done continuously toward the replication fork, but on the lagging strand this is done piecewise creating segments called Okazaki fragments. At this point DNA Polymerase I cleaves out the RNA primers and replaces them with DNA, and ligase reconnects any nicks between fragments. 24. D 25. K 26. E 27. H 28. J 29. G 30. L 31. B 32. A. Northern Blot B. Western Blot C. Southern Blot 33. 34. O 35. L 36. I 37. M 38. J 39. B 40. F 41. G 42. K 43. A 44. E 45. P 46. D 47. A 48. C 49. C 50. A 51. B 52. A...
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This note was uploaded on 01/19/2012 for the course PCB 3063C taught by Professor Mattgilg during the Fall '10 term at UNF.

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