10.27.2011 - with the 3 O of exon 1. Self-Splicing Introns...

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Lecture 15 October 27, 2011 RNA Transcription and Processing Pt. II (Ch. 8)
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TFIID: TBP + TAFs Six GTFs: TFIIA, B, F, E, H PIC = 6 GTFs + RNA Pol II RNA Pol II: 14 subunits General transcription factors
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β -subunit of RNA pol II GTF-dependent phosphorylation
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Processing and Termination: SEE THE PDF OF THIS LECTURE FOR THE LIST
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RNA Processing:
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Polyadenylation signal: AAUAAA or AUUAAA Cleavage 20 bp 3’ of Poly(A) signal Poly(A) tail added: 150-200 adenine nts
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Introns removed from primary transcript Introns vary in number and in size from gene to gene Co-transcriptional
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Alternative splicing:
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Intron removal and exon splicing: 5’ exon 3’ exon
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The splicing machinery: Large complex of small RNAs and proteins
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Exon splicing reactions:
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R C O OR’ Ester Reaction 1: the ester bond between the 5’ P of the intron and the 3’ O of exon 1 is exchanged for an ester bond w/ the 2’ O of the branch-site A. Reaction 2: the ester bond between the 5’ P of exon 2 and the 3’ O of the intron is exchanged for an ester bond
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Unformatted text preview: with the 3 O of exon 1. Self-Splicing Introns An intron that can undergo splicing w/o the addition of any proteins An example of RNA w/ catalytic activity (ribozyme) RNA world Self-splicing reaction (Part 1) Attack by G on the 5 splice site, adding G to 5 end of intron and releasing 1st exon Self-splicing reaction (Part 2) First exon attacks 3 splice site, ligating the two exons and releasing the intron Small RNAs: miRNAs and siRNAs Regulate gene expression Maintain stable genome miRNA Discovered in 1993 by Victor Ambros lin-4 gene required for the development of normal larvae Argonaute 2 (slicer) cleaves target mRNA With siRNA: siRNA siRNA silences the gene that produces it. Shuts off genetic elements that insert into the genome (e.g., viruses, transposons) Cosuppression of transgene and endogenous gene: miRNAs siRNAs piRNAs (Discussion) Short RNAs typed based on their biogenesis:...
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This note was uploaded on 01/19/2012 for the course BIOLOGY 305 taught by Professor Kumar during the Fall '11 term at University of Michigan.

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10.27.2011 - with the 3 O of exon 1. Self-Splicing Introns...

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