11.08.2011 - Lecture 18 November 8 2011 Gene Isolation and...

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Lecture 18 November 8, 2011 Gene Isolation and Manipulation (Ch. 10) Regulation of Gene Expression in Prokaryotes (Ch. 11)
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Analyzing DNA: Southern Blotting: Large DNA fragments DNA Sequencing: Determining the DNA nt sequence
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You are analyzing the following locus using Southern blotting: EcoRI EcoRI EcoRI 5 kb 3 kb 1. Digest DNA with EcoRI 2. Run on gel 3. Transfer DNA to membrane 4. Hybridize with radioactive probe Probe 1 kb 4 3 2 5 6 Probe 1 Analyzing genomic DNA by Southern Blotting:
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The diagram below shows the map of a gene; boxes represent exons, and lines represent intergenic regions. Xba I sites and the distances between them are shown. A region corresponding to a probe used for Southern blotting is shown. In allele b, the indicated region is deleted. In allele c, the second Xba I site is mutated from AGATCT to AGAACT. Kate has genotype b/+; Mark has genotype c/c. Indicate the lanes containing Kate’s DNA and Mark’s DNA. iClicker Question 1:
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High-Throughput / Next-Gen Sequencing: 454 Life Sciences Founded as subsidiary of Curagen Corp.
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