11.10.2011 - Lecture 19 November 10, 2011 Regulation of...

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Lecture 19 November 10, 2011 Regulation of Gene Expression In Prokaryotes (Ch. 11)
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lac genes F’ lac genes Chromosomal DNA cis : only affects gene expression on the same piece of DNA sequence Example: promoter, operator trans : diffusible, affects expression of genes in the whole cell Example: proteins (repressor, activator) Partial diploid cis and trans- acting elements:
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iClicker Question 1: Using your understanding of the lac operon as a guide, how would you characterize the following mutation in gene X from an unrelated operon? You have isolated a mutation in gene X that yields a defective protein product that can still form a dimer w/ wild-type repressor monomer; however, the defective protein binds the repressor very effectively and prevents the wild-type repressor from binding the operator. You expect this mutation to be: A) Trans-acting, dominant negative B) Trans-acting, recessive C) cis-acting, recessive D) cis-acting, dominant negative
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: presence of glucose prevents expression of lac operon genes; true for many catabolic operons Expression of genes in the operon will only be induced in the absence of glucose. Catabolite Repression:
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11.10.2011 - Lecture 19 November 10, 2011 Regulation of...

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