12.01.2011 - Lecture 24 December 1, 2011 DNA Repair,...

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Lecture 24 December 1, 2011 DNA Repair, Recombination, and Cancer Pt. II (Ch. 16)
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Mismatch Repair: Replication errors are corrected by 3’-5’ proofreading of the replicative polymerase; mismatch repair corrects the remaining errors Three Steps: 1. Recognize mismatch 2. Determine incorrect base in mismatch 3. Excise incorrect base; repair
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Mismatch Repair in E. coli MutS MutL, MutH Adenine methylation
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Error-Prone Repair Error-prone repair allows for the bypass of stalled replication forks by inserting nonspecific bases “Bypass” polymerases are found in eukaryotes and prokaryotes; the SOS system for error-prone repair is found in E. coli
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SOS System: UV light induces synthesis of RecA RecA protein-DNA filament; leads to the induction of bypass pol
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Bypass Polymerase: Tolerate large adducts; lacks 3’-5’ proofreading; falls off DNA quickly
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Rad6 adds ubiquitin monomer to PCNA; conformational change allows binding of bypass polymerase; resulting base mismatches corrected by mismatch repair
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12.01.2011 - Lecture 24 December 1, 2011 DNA Repair,...

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