MakRecDNA

MakRecDNA - -might be called molecular scissors 2. DNA...

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MAKING RECOMBINANT DNA I. Vectors: - carry foreign genes 1. Plasmids – small accessory rings of DNA taken from bacteria 2.Viral DNA – to carry recombinant DNA into a cell 3.Viral RNA – is best for mammalian cells (Retroviruses) II. ENZYMES To cut and seal 1 1. Restriction Enzymes - named because they’re found in bacteria, restrict growth of invading viruses - cut DNA at specific, predictable locations called restriction sites leaving single strands exposed known as sticky ends
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Unformatted text preview: -might be called molecular scissors 2. DNA Ligase 2 - seals any breaks in DNA molecule 3 - seals foreign DNA into the vector 4- might be called molecular glue III. Producing Foreign Proteins- Once recombined plasmid (virus) has been taken up and the gene functions normally, cell has been transformed - It produces a protein product it never produced before- Either the cloned gene or its protein product may be recovered...
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This note was uploaded on 01/17/2012 for the course BIOL 103 taught by Professor Robertwest during the Spring '12 term at Lee.

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MakRecDNA - -might be called molecular scissors 2. DNA...

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