3.AtomicSpectra

3.AtomicSpectra - • No theoretical understanding of equations Bohr Model Applications of Atomic Spectra • Elemental fingerprint • In theory

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Atomic Spectra Week 3 Lab S117 Fall 2011
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Overview Post-Lab: MS Modern Mass Spectrometry techniques Analysis of Proteins Informatics Pre-Lab: Atomic Spectra Theory Application of atomic spectroscopy Practical aspects
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Modern Mass Spectrometry Inlet System Ion Source Mass Analyzer Detector Vacuum (10 -5 10 -8 torr)
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Modern Mass Spectrometry Double focusing instruments GC-MS MALDI-TOF Electrospray
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Identification of Proteins Purified Protein Mix of Peptides Trypsi n Digest Mass Data of mixture Mass Spec MS Librar y Identity of Protein ASRLVMKQQKD ASR LVMK QQK D Example 1 Example 2 ASRLVKMQKQD
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Informatics Applied information technology The data is there—you just have to know how to organize and find it! Genomics Proteomics
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Atomic Spectroscopy Problem of line spectra Empirical series of numbers Predicted by equations
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Unformatted text preview: • No theoretical understanding of equations Bohr Model Applications of Atomic Spectra • Elemental fingerprint • In theory, just have to get atoms apart and pumped full of energy —then measure the fingerprint of line spectrum emitted or absorbed • Practice is tougher than principle! Bunsen and Kirchhoff would be proud. This Week in Lab • Collect electric emission data from 6 elements/compounds • Collect flame emission data from 6 ions • Use data to determine difference in energy levels and identify two unknowns Considerations • Discharge lamps: HIGH VOLTAGE – Do not touch unless unplugged – Do not stare at light – 30s on/30s off • Use gloves with salts • Turn off Bunsen burners when not in use • Use correct Nichrome wires...
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This note was uploaded on 01/17/2012 for the course S 117 taught by Professor Stephenjacobson during the Fall '11 term at Indiana.

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3.AtomicSpectra - • No theoretical understanding of equations Bohr Model Applications of Atomic Spectra • Elemental fingerprint • In theory

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