{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

3.AtomicSpectra

3.AtomicSpectra - • No theoretical understanding of...

Info icon This preview shows pages 1–11. Sign up to view the full content.

View Full Document Right Arrow Icon
Atomic Spectra Week 3 Lab S117 Fall 2011
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Overview Post-Lab: MS Modern Mass Spectrometry techniques Analysis of Proteins Informatics Pre-Lab: Atomic Spectra Theory Application of atomic spectroscopy Practical aspects
Image of page 2
Modern Mass Spectrometry Inlet System Ion Source Mass Analyzer r Detector Vacuum (10 -5 10 -8 torr)
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Modern Mass Spectrometry Double focusing instruments GC-MS MALDI-TOF Electrospray
Image of page 4
Identification of Proteins Purified Protein Mix of Peptides Trypsi n Digest Mass Data of mixture Mass Spec MS Librar y Identity of Protein ASRLVMKQQKD ASR LVMK QQK D Example 1 Example 2 ASRLVKMQKQD
Image of page 5

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Informatics Applied information technology The data is there—you just have to know how to organize and find it! Genomics Proteomics
Image of page 6
Atomic Spectroscopy Problem of line spectra Empirical series of numbers Predicted by equations No theoretical
Image of page 7

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 8
Image of page 9

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 10
Image of page 11
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: • No theoretical understanding of equations Bohr Model Applications of Atomic Spectra • Elemental fingerprint • In theory, just have to get atoms apart and pumped full of energy —then measure the fingerprint of line spectrum emitted or absorbed • Practice is tougher than principle! Bunsen and Kirchhoff would be proud. This Week in Lab • Collect electric emission data from 6 elements/compounds • Collect flame emission data from 6 ions • Use data to determine difference in energy levels and identify two unknowns Considerations • Discharge lamps: HIGH VOLTAGE – Do not touch unless unplugged – Do not stare at light – 30s on/30s off • Use gloves with salts • Turn off Bunsen burners when not in use • Use correct Nichrome wires...
View Full Document

{[ snackBarMessage ]}