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4.BioanalyticalApplicationofUV

4.BioanalyticalApplicationofUV - Bioanalytical Application...

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Bioanalytical Application of UV/vis Spectroscopy Week 4 Lab S117 Fall 2011
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Overview Post-lab Discussion Historical Perspective on Balmer, Rydberg, Bohr How science builds Pre-lab discussion Molecular Spectroscopy UV-vis Spectrometer Quantitative use of spectroscopy Standard curves
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Balmer’s Contribution Experimental Empirical observation of a series Mathematical description of series n2 n2 - 22 Wavelength = constant *
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Rydberg’s Contribution Generalization Recognizing use of inverse wavelength
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Bohr’s Contribution Theoretical Assume classic interpretation BUT…only if angular momentum restricted by quantum rule From classical treatment mixed with quantum rule:
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Why is Bohr Accepted? Direct match to experimental results Explains Rydberg in terms of more fundamental constants Philosophical question: How right is a “right theory” and how wrong is a “wrong theory?”
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Pre-lab: UV/vis Spectroscopy
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  • Fall '11
  • StephenJacobson
  • Spectroscopy UV-vis Spectrometer, Approximate maximum wavelength, spectroscopy Standard curves, Lamp Wavelength Selector

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