EnzymeKinetics

# EnzymeKinetics - Enzyme Kinetics Law Depends on Mechanism...

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12/6/2011 1 Enzyme Kinetics Law Depends on Mechanism Remember our previous example If first mechanistic step is rate determining, rate law can be written from first step O 3 + 2 NO 2 O 2 + N 2 O 5 1. O 3 + NO 2 NO 3 + O 2 slow 2. NO 3 + NO 2 N 2 O 5 fast Rate = k[O 3 ][NO 2 ] More Complex Mechanism What if the second step is rate limiting? What if RDS is unknown? Using the Steady State Approximation , we can write a general rate law and determine which RDS fits the experimental evidence Your book presents steady state and other techniques, but steady state assumption works well for all situations generally encountered

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12/6/2011 2 Breakdown of Ozone O 3 O 2 + O O + O 3 2O 2 Rate = k 2 [O][O 3 ] but O is an intermediate. How do we determine [O]? k 1 k 1 k 2 Steady State Approximation Assumption: concentration of intermediates stays about constant during reaction Low, constant [Intermediate] Rate of appearance = rate of disappearance For this mechanism: O 3 O 2 + O O + O 3 2O 2 k
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