S117Lecture112011 - Interaction of Light(Electromagnetic...

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Molecular Spectroscopy We will discuss: Electronic spectroscopy Vibrational spectroscopy Rotational spectroscopy Nuclear Magnetic Resonance (NMR) spectroscopy Spectroscopy can teach us about the structure of a molecule. It can be used to identify the presence of certain bonds in a molecule. It can also be quantitative. Spectroscopy is non destructive. You can subject the sample to repeated interrogations of the same type–or a different type. Internal structure of molecules Electronic  UV/VIS Vibration  Infrared (IR) Rotation  Microwave NMR  Radiofrequency Interaction of Light (Electromagnetic Radiation) with matter
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Electronic transitions Involves a transition from one electron arrangement to another. Typically, electronic transitions require photons in the ultraviolet or visible portion of the EM spectrum. (This is what you are doing with your UV-VIS spectrophotometer). Accurate method to determine the amount of an absorbing species in a sample
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S117Lecture112011 - Interaction of Light(Electromagnetic...

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