330outlineintro08 - D. Cell-Cell Contacts/Cell Biology E....

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I. Introduction II. What is a virus? III. Structure of Viruses A. Morphology/Structure 1. Icosahedral Capsids - Equivalence and Quasi-Equivalence - 5:3:2 Rotational Symmetry - Triangulation Numbers 2. Helical Capsids 3. Complex Morphology B. Viral Nucleic Acids C. Envelope IV. Classification A. Properties Used for Classification B. ICTV Classification V. Methods for Studying Viruses – The Plaque Assay B. Animal Virus Plaque Assay - Centers of Infection C. Dose Response – Linear response to virus dose for most replication-competent animal viruses VI. Tissue Culture A. Conditions for Cell Growth B. Primary and Secondary Cultures C. Cell Strains/Cell Lines
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Unformatted text preview: D. Cell-Cell Contacts/Cell Biology E. Cell Membranes VII. Cellular Transformation A. Focus-Forming Assay B. Saturation Density C. Anchorage Independence D. Reduced Serum Requirement E. Immortalization F. Alteration in Surface Properties G. Tumorigenicity in Animals H. Blocks to Differentiation VIII. The Infectious Cycle Productive Infection A. Adsorption B. Penetration/Uncoating 1. Enveloped Viruses 2. Non-enveloped Viruses 3. Uncoating C. Replication of RNA Viruses 1. Overall Strategies 1 2. Classes of RNA Viruses D. Replication of DNA Viruses E. Maturation/Release 1. Naked Icosahedral Viruses 2. Enveloped Viruses 2...
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330outlineintro08 - D. Cell-Cell Contacts/Cell Biology E....

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