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4_7_08 - Lecture 3 DNA and Chromosomes Outline Chromosome...

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Lecture 3 DNA and Chromosomes
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Outline Chromosome Positioning and Looping Global Structure of Chromosomes Comparative Genomic Analysis Chromosome Analysis in Cancer Cells
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05_24_Chromatin pack.jpg
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Fluorescent In Situ Hybridization (FISH) Do chromosomes occupy distinct domains in nucleus? P. Lichter et al., 1988, Hum Genet. 80: 224 How could you test this? Fix cells Hybridize with mixture of fluorescent DNA probes specific for sequences along an entire chromo- some Visualize by fluorescence microscopy
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How do we know that chromatin forms loops in vivo ? FISH with fluorescently labeled DNA probes complementary to specific DNA sites located at known distances along a chromosome Expected results if chromatin forms loops? Does not form loops? What control DNA could you compare your results to? Fig. 10-25 Lodish et al., 2004
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What cellular processes will be affected by chromatin?
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Summary Evolution of eukaryotes from ancient prokaryotes allowed specialization and opportunities for regulation
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  • Spring '08
  • UCHWITZ,BRIANJOSEPH
  • DNA, comparative genomic analysis, Chromosomes Comparative Genomic Analysis, fluorescent DNA probes, Chromosomes Comparative Genomic

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