section9

section9 - MIT Department of Biology 7.014 Introductory...

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MIT Department of Biology 7.014 Introductory Biology, Spring 2005 Recitation Section 9 March 7-8, 2005 Molecular Biology—Gene Regulation A. Gene regulation basics 1. What is a gene? 2. What is a protein? 3. How is it possible that your liver cell and your skin cell can have identical DNA content, and yet look different and perform different functions? 4. Why is there a need for gene regulation? In general terms, how is it accomplished? 5. What does a gene consist of? What is the role of each element?
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6. What regulates the expression of a gene? 7. What are the cis- and trans-acting elements? 8. Can a deficiency in a trans-acting element be overcome by the addition of another copy of the gene to a cell? What about a cis-acting element? Why? 9. What proteins will be affected by mutations in the trans-acting elements? Cis-acting elements? B. Gene regulation—an example The imaginary bacterium E. fictionalis requires two enzymes for the metabolism of the imaginary sugar froyose. Enzyme 1 converts froyose to sweetose; enzyme 2 converts sweetose to glucose.
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section9 - MIT Department of Biology 7.014 Introductory...

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