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Unformatted text preview: 7. What is in complete medium? 8. What is in minimal medium? III. Planning the experiment 9. If you wanted to make media on which to grow a strain that was unable to synthesize cysteine, what would you need to put in it? 10. What medium could we use to screen for cysteine auxotrophs? 11. What is a selective condition? 12. Why don’t we plate the mutagenized yeast directly on selective conditions? 13. How should we find the rare mutant yeast that cannot synthesize cysteine within the large collection of yeast colonies we have on the plates? IV. Identifying mutants 14. When you “screen” the plates, how do you identify the mutants? 15. If we find some colonies that grow on CM but not MM, can we determine which amino acid they are unable to synthesize? 16. How might we figure this out?...
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