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section11 - MIT Department of Biology 7.014 Introductory...

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MIT Department of Biology 7.014 Introductory Biology, Spring 2005 Recitation Section 11 March 14-15, 2005 Bacterial Genetics—Complementation and Epistasis A. Understanding the basis Working in groups, choose the best answer to the questions below. Justify your choices. 1. A complementation test involves crossing a. wild-type organisms with recessive mutant variants. b. two different wild-type strains c. two strains displaying the same recessive mutant phenotype d. two strains displaying different recessive mutant phenotypes 2. Auxotrophy strains that ____complement____ each other will grow on minimal media. _do not complement_ 3. A complementation test determines whether a. two pieces of DNA in the two strains interact with each other b. two pieces of DNA in the two strains are identical to each other c. products of expression of the gene(s) of interest in the two strains can interact d. products of expression of the gene(s) of interest in the two strains are variants of the same protein e. DNA from one strain interacts with the protein from the other 4. A complementation test a. determines the identity of the genes in a pathway. b. helps resolve the number of genes in a pathway. c. determines the epistatic order of genes in a metabolic pathway. d. resolves the number of genes in a pathway.
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5. All organisms in the same complementation group have identical DNA sequence.
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