section17 (1)

section17 (1) - MIT Department of Biology 7.014...

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MIT Department of Biology 7.014 Introductory Biology, Spring 2005 Recitation Section 17 April 11-12, 2005 Recombinant DNA and Cloning A. The Tools Recombinant DNA is a set of tools that allows scientists to move between genetics, biochemistry and molecular biology – allowing us to determine how the parts of a cell or organism work. 1. What does it mean to clone a gene? 2. What is a vector? 3. What do the restriction enzymes do? How are they physically able to perform this function? 4. What is a library? 5. What three features must vectors or plasmids have? Why? 6. Where did the techniques and reagents used in recombinant DNA applications come from? 7. What is the purpose of the recombinant DNA technology? 1
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B. Application You have been given a purified DNA preparation of pN1, a 3900bp circular plasmid, which contains a bacterial origin of replication (ori) and the gene for kanamycin resistance (kan r ). In addition, you also have a preparation of an 800bp linear DNA fragment (isolated from a HincII restriction digest of DNA from another source) that contains the entire gene for DsRed (a red fluorescent protein). These two
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section17 (1) - MIT Department of Biology 7.014...

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