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section22 - MIT Department of Biology 7.014 Introductory...

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MIT Department of Biology 7.014 Introductory Biology, Spring 2005 Recitation Section 22 May 2-3, 2005 From Yeast to Humans—Essential Genes on the Evolutionary Continium. A. CBS protein in yeast and humans Recall that earlier in the term we considered the human gene CBS and its yeast analog cys4. Recall that these genes each encode the protein cystathionine β -synthase that is responsible for converting homocysteine into cystathionine in the cellular pathway of creating cysteine. 1. What is cysteine? Is it important for organism’s survival? 2. What would you expect to be the result of complete absence of the protein product of the yeast CYS4 gene to be? What about the same question for the human CBS protein? 3. Would you expect cells that contain no functional copy of CBS enzyme to accumulate some kind of a compound? If no, why not? If yes, what kind of a compound would you expect that compound to be? 4. In the experiments we discussed earlier in the term, what was the phenotype of the cys4 mutants on complete media?
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