Biochemistry 461FS 2009Michigan State UniversityProtein PurificationSeptember 9, 2009Lecture 3Protein PurificationGeneral strategy for protein purification (5 Basic Steps)1.Rupture cells to release proteins•Freeze•Sonicate•French press (not for coffee)•Vortex with small glass beads2.Collect the proper fraction3.Purify protein of interest by selecting for characteristics of that protein4.Determine purity of protein – Is protein pure?•No? Repeat step 3•Yes? Continue5.Characterize purified protein2
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