2009-09-28_-_Lecture_10

2009-09-28_-_Lecture_10 - Biochemistry 461 FS 20089...

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Biochemistry 461 FS 20089 Michigan State University Introduction to Enzyme Kinetics September 28, 2009 Lecture 10 Kinetics can tell you about mechanism • Disprove mechanisms that are not consistent with the data. • Propose a reaction mechanism that is consistent with the data. • Gain insight into the role of particular amino acid residues. (E.G., “Arg XXX is not involved in substrate binding but is critical for efficient reaction catalysis.”) Why Study Enzyme Kinetics? 2
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• Study reaction velocity as a function of: • Enzyme concentration -- usually not very informative • Temperature -- determine activation energies • pH -- acid/base catalysis Substrate Concentration(s): -- this is the central tool • Normally measure the initial rate ( ν 0 ), to avoid complications • Normally [S] >> [E] How Do You Study Enzyme Kinetics? 3 Michaelis and Menten interpreted the plots of ν 0 vs. [S] in terms of a reaction scheme centered on the enzyme-substrate complex (ES) which is sometimes called the Michaelis complex . They proposed: • The formation of an ES complex is a necessary step • The first step is S binding to E and that this step is a relatively fast and reversible step
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This note was uploaded on 01/17/2012 for the course NSC 461 taught by Professor Benning during the Fall '11 term at Michigan State University.

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2009-09-28_-_Lecture_10 - Biochemistry 461 FS 20089...

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