ANS Paper2 - Methods and Materials The ten different...

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Methods and Materials The ten different litters of mice were kept in the UC Davis Animal Science Teaching Facility in plastic cages. The CD1 outbreed mouse was used in this experiment. Individual mothers and their litters were kept separate from the other litters. The mother’s were naturally mated and separated into individual cages prior to parturition. They were fed a standard commercial mouse diet and housed on a fixed light dark cycle. Each animal was cared for according to AALAC standards. The mice pups from each litter were weighed daily starting at one day after birth until weaning at day 21. At weaning age, the mice pups were separated into separate containers according sex. Each day after the separation, the litters were weighed from weaning to puberty, with the total weights of males and females recorded separately. Given an excel spreadsheet with collected data of each mother’s litter weights from birth to puberty, the two specific litters of mice were examined. Mouse four and mouse eight had the closest ratio of female to male offspring, so their data was used to determine the different effects of growth rates in a specific litter by comparing the weights and genders of litter four and litter eight, as well as the average weight and percent growth of each pup in before they were separated. Analysis of the data was done from the same period for each litter. The offspring mice pups of mouse four were weighed and observed daily after birth, March 31, 2010, until puberty. Mouse eight’s parturition date was April 1, 2010. Her mice Pups also began being weighed after their parturition date. Results Analysis of the mice pups’ growth was collected using two litters based on their weight. The lowest, median, and largest weights were chosen from the total ending weights of all the data collected.
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Litter four had fourteen pups and had a lower total ending weight than mouse eight. They were born on March 31, 2010, with a total weight of 27.33g which was 7.9% of their ending weight. By day three the pups weighed 9.5% of their ending weight at 33.09g, averaging 2.36g per pup. On day six the pups weighed 50.49g which was 14.6% of their ending weight. By day nine the litter increased their weight to 65.13g which was 18.8% of their ending weight. The pups weighed 77.38g on day twelve which was 22.3% of their ending weight. The litter weighed 85.48g on day fifteen which was 24.6% of their ending weight. Day eighteen the pups weighed 95.08g which was 27.4% of their ending weight. Their ending weight for analysis containing both female and male offspring was 129.74g which was 37.4% of the total weight gained by each
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This note was uploaded on 01/17/2012 for the course ANIMAL SCI 2 taught by Professor Murphy during the Spring '10 term at UC Davis.

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ANS Paper2 - Methods and Materials The ten different...

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