MICRO 06 Lect 3

MICRO 06 Lect 3 - LECTURES IN MICROBIOLOGY Laboratory Tools...

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Laboratory Tools in Microbiology Sofronio Agustin Professor LECTURES IN MICROBIOLOGY LESSON 3
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Topics Covered Microscopy Staining Techniques Methods of Culturing Microbes
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Units of Measurement 1 µm = 10 -6 m = 10 -3 mm 1 nm = 10 -9 m = 10 -6 mm 1000 nm = 1 µm 0.001 µm = 1 nm
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Simple Microscope A simple microscope has only one lens
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Compound Microscope A compound microscope has two sets of lenses and is typically used in teaching and research laboratories. Parts of a student laboratory microscope
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Optics : Magnification A specimen is magnified as light passes through the objective and ocular lenses. Total Magnification = objective lens X ocular lens (magnifications) The pathway of light and two stages of magnification of a compound microscope.
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Optics: Resolution Resolution is the ability of the lenses to distinguish two points. A microscope with a resolving power of 0.2 um can distinguish between two points > 0.2 um. Resolution distinguishes magnified objects clearly.
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Optics: Resolution Resolution can be increased by using immersion oil and shorter wavelengths of light.
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Optics: Refraction Refractive index is the light- bending ability of a medium. The light may bend in air so much that it misses the small high-magnification lens. Immersion oil is used to keep light from bending.
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Brightfield Microscopy Dark objects are visible against light background. Used to observe stained or unstained specimens. Most commonly used in laboratories.
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Light objects are visible against a dark background. Light reflected off the specimen enters the objective lens.
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This note was uploaded on 01/18/2012 for the course MCB 2010C taught by Professor Sofronioagustin during the Fall '06 term at Valencia.

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MICRO 06 Lect 3 - LECTURES IN MICROBIOLOGY Laboratory Tools...

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