MICRO 06 Lect 71

MICRO 06 Lect 71 - LECTURES IN MICROBIOLOGY Control of...

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Control of Microbial Growth Sofronio Agustin Professor LECTURES IN MICROBIOLOGY LESSON 7
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2 Lesson 7 Topics Controlling Microorganisms: Physical Means Chemical Means
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3 Overview Various Microbial Control Methods
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4 Antimicrobial Action -static agents- inhibit growth of microbes Ex: bacteriostatic, fungistatic -cidal agents - destroy or kill microbes Ex: bactericidal, fungicidal
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5 Terms Sterilization Disinfection Antisepsis Sanitation Degerming
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6 Sterilization Complete removal or destruction of all viable microbes including endospores. Application: Surgical instruments and commercially packaged foods. Commercial sterilization : Killing C. botulinum endospores.
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7 Disinfection Use of physical or chemical agents (disinfectant) to kill vegetative bacteria and other microbes except endospores. Example: 10% Bleach solution on bench tops.
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8 Antisepsis Use of physical or chemical agents to kill pathogens on living tissue. Example: Scrubbing surgical patients with chemical agents.
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9 Sanitization Reduction of microbial counts to acceptable levels of public health standards. Example: Sanitization of eating utensils in restaurants.
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10 Degerming Physical removal or reduction of microbes from a limited area. Ex: Washing of hands with germicidal soap.
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11 Efficacy Depends On Number of microbes Target microbe characteristics Environmental factors - temperature, pH, biofilms Concentration and mode of action of agent Organic matter - sputum, blood, feces
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12 Factors Factors that influence the effectiveness of antimicrobial agents
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13 Mode of Action Damage to cell wall Alteration of membrane function Damage to proteins Damage to nucleic acids
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14 Damage to Cell Wall Effects on bacteria and fungi: Blockage of cell wall synthesis Degradation of cell wall components Reduction of its stability and integrity Ex: Penicillin, detergents, alcohols
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15 Injury to Cell Membrane Effects on microbes including enveloped viruses: Bind and penetrate membrane lipids Loss of selective permeability resulting in leakage of cytoplasmic contents. Ex: S urfactants - surface active agents.
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16 Surfactant Action The effect of surfactants on the cell membrane.
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17 Effects on Nucleic Acids Irreversible binding to microbial DNA results in: Ceasation of transcription and translation Mutations Ex: Formaldehyde and ionizing radiation
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Effects on Proteins Blockage of enzyme active sites prevents binding of substrate. Protein denaturation.
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MICRO 06 Lect 71 - LECTURES IN MICROBIOLOGY Control of...

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