Bacterial_Transformation_Data_Analysis - Prabhleen Kaur Adv...

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Prabhleen KaurAdv. Bio 2February 25, 2019Bacterial Transformation Data AnalysisIn this lab, we took E.coli bacteria and attempted to genetically transform it to carry the gene for green fluorescent (PGlo gene from Aquorea Victoria), and the ampicillin resistance geneby injected a plasmid into it. We had a total of 4 LB bacterial growth plates, two of which were control and the other two were injected with the plasmid. There were also two plates that had ampicillin, a bacteria killing antibiotic in them (labeled LB Amp- and LB Amp+). For the LB- plate, we hypothesized that “If bacteria given no plasmid are grown on the agar with no ampicillin, then there will be lots of bacteria growth but no green fluorescent because the plasmid wasn’t inserted for glow and antibiotic resistance, and the ampicillin would kill all of thebacteria.” For the LB+ plate, we hypothesized that “If bacteria given the plasmid are grown on

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