MCDB Christoffersen Lecture#8

MCDB Christoffersen Lecture#8 - MCDB Christoffersen Lecture...

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MCDB Christoffersen Lecture #8 Trinucleotide Repeat Diseases Expanding triplet repeats have been found in other diseases: Myotonic dystrophy, Huntington’s disease How the repeats expand is unknown o Possibly DNA polymerase slips after copying the triplet, and copies it again Genetic Screening DNA testing is direct analysis of DNA for mutation; the most accurate way of detecting an abnormal allele Preimplantation screening of zygote can be used for parents of a child with a disease like cystic fibrosis. Fetal cells and newborns can be tested for sickle-cell disease and others. Screening for Sickle-Cell Allele The allele-specific cleavage method of DNA testing uses RFLP analysis In the ß-globin gene the sickle-cell allele doesn’t contain the enzyme recognition sequence for the restriction enzyme Mstll, which will not be cut PCR products derived from the normal and the sickle allele will differ in gel electrophoresis. Genetic Markers
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This note was uploaded on 01/19/2012 for the course MCDB 1a taught by Professor Feinstein during the Fall '08 term at UCSB.

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