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MCDB Christoffersen Lecture#10

MCDB Christoffersen Lecture#10 - MCDB Christoffersen...

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MCDB Christoffersen Lecture #10 The lac Operon of E. coil and its Regulator There are three sites to make three different kinds of proteins The operator and promoter play a key role with the protein. The sequence of DNA of both the operator and promoter has a sequence of DNA only recognized by a specific protein The regulatory gene codes for repressor protein are expressed by a 2 nd gene produce a repressor protein Lactose Absent o There are active repressor which binds to the operator and prevents transcription of the lacto-operon Lactose Present o The lactose is an inducer, which binds to the repressor to make it inactive. Once inactive the RNA polymerase starts transcription. Summary of Lac-Operon http://bcs.whfreeman.com/webpub/biology/sadavalife9e/animated %20tutorials/life9e_1601_script.html The trp Operon: A Repressible System Turns off genes you don’t need, because some metabolites are not required all the time. There are some metabolites required at all times Example: Amino Acids are need to charge tRNAs are needed at all times. If any amino acid-tRNA is missing, protein synthesis stops when ribosome is unable to insert correct amino acid Cell expresses genes needed to synthesize amino acid if it is not available from the environment If amino acid level is adequate, then it is more efficient to not express genes.
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