Study guide for Exam 3 - Study guide for Exam 3 Chapters:...

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Unformatted text preview: Study guide for Exam 3 Chapters: 12 (partly), 13, 14, 15 Chapter 12: Bacterial gene regulation: 1) Understand the regulation of the trp operon by the trp repressor at transcription initiation. 2) Trp operon is also regulated at transcription elongation understand the formation of terminator and anti-terminator loops and how presence/absence of trp influences their formation thereby promoting/inhibiting transcription. 3) Explain how AraC acts as an inhibitor and an activator in regulating the Ara operon. 4) What are riboswitches? What is their role in gene regulation? ribosomes are not involved a specialized form of transcription attenuation folding of leader sequence (the riboswitch) determines if transcription will continue or be terminated folding pattern altered in response to binding of an effector molecule in gram negative bacteria regulate the translation of mRNA blocking of SD sequence 5) What constitutes a regulon? What are some of the mechanisms used for global regulation? genes or operons controlled by a common regulatory protein global regulatory systems often use many types of regulation such as: regulatory proteins alternative sigma factors two component signal transduction systems Phosphorelay systems 6) What is Quorum sensing? Know N-acyl-homoserine lactone a signal molecule in some Gram negative bacterial Cell to cell communication mediated by small signaling molecules N-acyl-homoserine lactone (AHL) in several gram negative bacteria Required for coordination of gene expression in population Couples cells density to transcription regulation Examples biofilms, virulence establishment, symbiosis etc 7) What are the components of the phosphorelay system that regulate gene expression in response to the environment (like temp, pH etc) the regulatory proteins involved are part of a two-component signal which links external events to regulation of gene expression Sensor kinase Response regulatory protein o Genes turned on/off in response Chapter 13: Plasmids and gene transfer: 8) What are plasmids? Genetic elements replicate independently ds DNA (exceptions linear) Carry unessential genes >300 naturally occurring plasmids in E.coli What are the different types of plasmids? F plasmid, R plasmids, Col plasmids, Virulence plasmids, metabolic plasmids What do these terms mean: Copy number Different plasmids present in cells in different numbers 1 3 copies or > 100 copies Controlled by genes on plasmid and by interactions with host Plasmid incompatibility Multiple different types of plasmids in same cell may not be compatible Incompatibility groups- same group plasmids related to each other Plasmid curing Elimination of plasmid from cells Cause: Inhibition of plasmid replication 9) Understand the mechanism of rolling circle replication 10) Know the characteristics of resistance plasmids, col plasmid, F plasmid discussed.10) Know the characteristics of resistance plasmids, col plasmid, F plasmid discussed....
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This test prep was uploaded on 04/07/2008 for the course BIO 226r taught by Professor Latham during the Spring '08 term at University of Texas at Austin.

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Study guide for Exam 3 - Study guide for Exam 3 Chapters:...

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