Histology 12-6

Histology 12-6 - Histology 12/6/11 *look up crypts of...

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Histology 12/6/11 *look up crypts of lieberkhund, panet cells Microscopy and special techniques Light Microscropy WHAT DOES IT ALLOW US TO STUDY - Bright Field Microscopy o If white light passes through things that are colorless, we don’t see an image (no contrast). Requires for parts of specimen to absorb and some to not. Contrast= intensity of difference between the two o Can be done with aperture size of diaphragm o Or stain it - Histochemistry o Changes color based on rxn. (PAS) - Immunochemistry o Primary and secondary antibodies o Secondary binds to target - Molecular Probe o Attach an enzyme or other marker to DNA sequence to probe for specific sequence o For differentiation of cell or maybe cancer - Autoradiography o Use radioactive things, such as nucleotides o Coat the specimen with film, and causes it to be exposed. Creates an image of where radioactive nucleotides, amino acids, etc. were - Dark Field o Puts it against dark background o Can highlight things like flagellum o Most efficient way to do it is to have condenser with an opaque disk, and only
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This note was uploaded on 01/21/2012 for the course BIO 413 taught by Professor Dr.donaldlovett during the Fall '11 term at TCNJ.

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Histology 12-6 - Histology 12/6/11 *look up crypts of...

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