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exam3_key - Name 1 What is the relationship between...

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Unformatted text preview: Name: 1. What is the relationship between evolution, natural selection, and genetic drift? Include in your answer a definition of each of the three terms. An example (hypothetical or real) may help but is not required. 20 pts. gfixokwkbhi bk Chqwk M— Mwewfj armam— rflews We v“: ”um / Tm; m 2. MCLHKSMS lel Walk-Arc, ddolew a} \ll- CL 540nm flames in (k W19: ad’ WP 09 .H‘L “41:4; was one a,“ lS‘w-«o‘ bi; man 0(a— 44gb Hart/k ””8 hurt Chaim y 'H'LJLL waww . L” WW' bid-0?; ‘7an 5" ‘H‘h- .Shbfiéruaj— MEV‘Q L/c 49"; %‘L¢ls EWBM} Name: 2. A student in our class suffers from GB disease, which is diagnosed by an inexplicable increase in intelligence and athletic ability. and is caused by an autosomal recessive allele. About one in 110,000 newborns are affected; those individuals born in the continental US usually retire to the island state of Hawaii by age 20. a. Assuming the locus is in Hardy-Weinberg equilibrium, calculate the frequency of the GB and the wild type alleles in the papulation. (10 pts) b. If the US has 313 million people, how many would be heterozygous (again given HW), and how many would be unaffected? (10 pts) 0. Which assumption of Hardy-Weinberg equilibrium would you think is most likely to be violated by GB disease, and why? (5 pts) d. Conduct a chi-squared test to see whether or not the locus is really in HW or not, if l tell you that in reality while 2,845 people are affected, 312,997,155 are not and of those unaffected people, none are heterozygotes. (10 pts) qt: code? = ‘lE-C ‘35,qu 91'2- 1. 1a,,~ O ‘1 ' «a -l 9723“) ~1 an 5-3:?) (Egg—I.— ,_ AA mama“ 3W1” GEE-€5’“ asthma“ ‘L cw ' Sigrid-wit"? :— teeusd dF=L 3. The following questions pertain to mutations. a. What is a mutation? (5 pts) [3. Why are mutations important for evolution? (5 pts) 0. What does each of these mutations do to the protein. if anything? What is the expected effect of each mutation on fitness? Note: these mutations are in codons that are in the middle of a protein required for the organism's survival. (15 points) codon CAU changes to CGU codon UUU changes to UUC codon UGU changes to UGA 0L . l9 muck-066m ES; 0A ddswfion vi: ~HAD. EMA Mama, a c/honOXL in OAK-OASIS. W“) 1303M. _>, QbUL hisfivca Aoq‘nim (Duck Misw/sg mwf‘dbe") 2)W&L—J§MML 5)u3u 4% ueA . . _. ‘ mm mm Whirl; #fifi/Em fimjdfiéfligfim 40AM: 14‘ Miss low” MW beginnirfi all MSG/{ig-h'on , H—~t C (A: laLVQ/L—f load-Rr-lvkzpo‘tem M55, LC H» 06.69”; MW- M, a m ma.“ ml- w 5.004.499 “ilk-OME- flDflwfié mufmhm, m5 W'ha WA . g g/C i‘HS'JIIIF-flaz, M3al-aW,‘f’VlIS cum/uni 196 had. Name: 4. How does the catabolite repression system (aka CAP-cAMP) of the lac operon function? What is in purpose? 15 pts. 1:? 31mm “.5 ram}, 50 :5 ms w+a£91.'i~es. We masks w {<0 aim“ flange, ms fem; up w mama MM: 56- erurfuon 69m. Lac Ofeyon is fmuemteol. m.“ Sum absenjr, M21111 oxide-4e M4 5W PM ATP, chck buds +0 CM Prawns. Tm, Complex M5 hear He (Phaml-d‘ fission 6? {-Le Lac open,“ ,‘dml‘aj H‘Wfifih} OP +113 Lac 33mg, 95 [whee can be m+abo‘iu3 «F0? are?” EFF—Eac-Lcra wofi‘l’ +0 (L‘I‘I L71 (BIU‘CW fmmmly our) IOC'l‘O‘ie ’I‘l‘)’ on 0.9234 +6 119615 Tim; impression syé'l-em modes 4 way ‘Gw bald-6M we? S‘ucose wLen manila“? on} Sun 31:". "‘3 I‘Cowfi ‘FOP energy WLUN alum 15 Y‘O‘I’ mk> U 5. Read the acrylamide gel below (i.e. provide the sequence of the DNA), which is the product of dideoxy sequencing (Sanger sequencing; 5 pts). Next, explain how gel electrophoresis works, including information about size of samples (2 pts), which end of the gel has which charge (3 pts). and why DNA migrates down the gel to the charge it does? (5 pts.) Finally, explain the role of dideoxynucleotides in Sanger sequencing (5 pts). Total: 20 pts. £1932” ddATP' dam: ddCTP screw ill D Y ' . &&ehfi mad-e 6U 741V Ag, - . /. mo . 0‘ their cmrla‘mg tire, veal-IS i5 mr'lLe/ *‘Hflfi- Sick). 04"ka .. M6319 I'll/1&5 E") "Cl/L 1 WA MQWWJW M? M m», . . hm. this amt «swam. in, paw/17 Lima 6:: n slat ‘Aia‘eOanz/léO‘l'i‘Aflj Lat/k q Bum-E OK A a”, vaJMi-Hn'r-lfiym‘wmzii cl {Vanawipfiom 4+ n gawk, Wei -- [AL For emmpfl -, -» - T w ddfil‘ilo _ S frT ccoene / (igfaw. [OWN x. 2—. ~$ mats an WWW Wmfifi g" TA > Hm _ . WSW \‘aéfiwfl' d . m M- Saw/“‘3’ . ' .H/wtf . WA’ lam/bu an a %[ fiz- heuiégflofi Name: 6. "The key to attenuation in the trp operon is that translation controls transcription.” What does this sentence mean? Be detailed. You may use a drawing if you like but be sure to label it carefully. 25 pts. Th 9P0 Emmott"), EG‘H" “PH“SC/P; Ff)?“ M491 WslM-fl’m ECU-{Ar W: W wince]. lb am “RNA lg [Wine lmscwwwi ;~—l mm £9653?» *msiw’cmh rm 9“ hbasam- U51“? “H43 +0? SPW'W M a.» CMM/vafi/ l'P tflWHiA’M 15 iwu ‘m wheelie? rm“ Cm fill/ob cell), lie ribaso‘w will Ari-S km tram-Elli“? my twmwh'm vi? a» '00? iv-Elwm 1w 5eCtM'9W‘“ com-BWE933’3 ivyei‘ome/w levels M im‘jk (Entitlmii), ‘H’bl nibodaw Ail-E) wi“ mmgmx. 1% Wide , lap-01135 will {larva Lflwée.h H7. / 3+4 amtivuj a. Slam be? poly U hit LWQK W- ‘l’t‘mtf‘lfkom wt“ flew-iWw ,- @ibsffim} WWW/w ls wwiwlllei 39V whys-M dfisfitwlwl “FRNRJE CW3” W at. WWW “Mai-W ”m“ ‘9‘” W. *9- +rE Malia (sixffil; BM Name: HAT H -— a o 7. Describe in detail how histone acetylaticn and deacety ation work to influence transcription. Please include in your definition any enzyme(s). what exactly is being acetylated, the effect of aoetylation on transcription. and speculate on why acetylation has this effect. (20 pts.) a??? cow. lac, Modlfiaftol ~Fmr men-iMflMaeSd‘k/t & ' I one, or; \ MW 'E‘VMMM‘IPLI‘WY‘ ‘97 “CC/JVTVA'TDW \ “15‘. 6" .\.7 q _ M t‘\ W Ware, M 39.5w, lads, JKWm‘i'gt/W‘ro UHF??? w\ “owx’l-Li \‘hfijroweh any“ {mew}, Pk, kaima. *\ (Ac/Q‘A’7AUA'I‘DHA 7 em ML -' '“M, Mall-M )l‘o {hem-fl. - was a 0‘10? - * \om 1553\97 3” 1“” 6‘4 Q JAWS; \ H 11 I 9N5” Wq’mfl 4"“W’W‘f WI . Name: 8. Please diagram single strand break recombination, starting after branch migration (i.e. from the drawing below), and showing both north-south and east-west resolution. The flanking markers. the A gene and the B gene, are in coupling as shown below. Show which resolution (east—west or north-south) causes recombination of the flanking markers, and which resolution causes a small heteroduplex region but not recombination of the flanking markers. 25 pts. Name: 9. The table below shows genotypes for a number of lac operons in which the 2 gene has been genetically engineered. The symbol zgfp represents an engineered gene that encodes a green fluorescent protein; cells producing this protein fluoresce green. The symbol zn‘p represents an engineered gene that encodes a red fluorescent protein; cells that produce this protein fluoresce red. Cells that produce neither protein do not fiuoresce at all, and those that produce both proteins fluoresce yellow. Complete the table by entering red. green, yellow, or none corresponding to the fluorescence of cells of each genotype when grown in the absence H or presence (+) of inducer. (15 pts.) .1. F’ro*z _/I 0 2w: . ...
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