Lecture 11 - Exam II Review

Lecture 11 - Exam II Review - Models for DNA Replication...

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Models for DNA Replication Semi-conservative model Conservative model Dispersive model
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Fig14.8 The Meselson-Stahl experiment
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Fig14.8 The Meselson-Stahl experiment Semi-conservative model - consistent with all data Conservative - eliminated after 1st generation Dispersive - eliminated after 2nd generation
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Sliding clamp holds DNA polymerase in place DNA polymerase III works in 5 3 direction, synthesizing leading strand RNA primer Leading strand 3 5 5 nthesis of leading strand begins. 5 3 Figure 14-11 SYNTHESIS OF LEADING STRAND
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Figure 14-12 Gap Forms in Lagging Strand Time 2 Time 1 3 3 5 5 5 5 5 5 5 3 3 3 3 Leading strand synthesized 5 3 3 Lagging strand synthesized 5 How is this growing gap filled in?
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Sliding clamp holds DNA polymerase in place DNA polymerase III works in 5 3 direction, synthesizing leading strand RNA primer Leading strand 3 5 5 nthesis of leading strand begins. 5 3 Figure 14-11 SYNTHESIS OF LEADING STRAND
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Figure 14-12 Gap Forms in Lagging Strand Time 2 Time 1 3 3 5 5 5 5 5 5 5 3 3 3 3 Leading strand synthesized 5 3 3 Lagging strand synthesized 5 How is this growing gap filled in?
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5 5 3 5 3 Okazaki fragment 3. Primase and DNA polymerase III synthesize another Okazaki fragment. 3 5 Okazaki fragment Figure 14-13 SYNTHESIS OF LAGGING STRAND
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12 A summary of DNA replication 5 3 Parental DNA 3 5 Overall direction of replication DNA pol III Replication fork Leading strand DNA ligase Primase OVERVIEW Primer DNA pol III DNA pol I Lagging strand Lagging strand Leading strand Leading strand Lagging strand Origin of replication Helicase Single-Strand Binding Proteins
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HOW TRANSCRIPTION BEGINS Promoter (on non-template strand) - 35 box - 10 box +1 site Upstream DNA Sigma Active site Downstream DNA RNA polymerase 1. Initiation begins Sigma binds to promoter region of DNA.
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2. Initiation continues Sigma opens the DNA helix; transcription begins. +1 site
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This note was uploaded on 01/21/2012 for the course BIOLOGY 172 taught by Professor Rajeshwarijanakiraman during the Spring '11 term at University of Michigan.

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Lecture 11 - Exam II Review - Models for DNA Replication...

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