Lecture 13 - 6:8 - Biology 172 Lecture 13: Wednesday, June...

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Lecture 13: Wednesday, June 8, 2011 Biology 172 Today’s Outline - Cancer Polymerase chain reaction (PCR) DNA sequencing - Genetic engineering in humans and plants Announcements Tomorrow’s Discussion: Stem cells Quiz on MONDAY’S and today’s lecture
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Figure 19-6 PCR primers must be located on either side of the target sequence, on opposite strands. 3 When target DNA is single stranded, primers bind and allow DNA polymerase to work. 5 3 3 3 3 3 5 5 5 5 5 Primer Primer Primer Primer Region of DNA to be amplified by PCR
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Figure 19-7-1 dNTPs Primers 5 2. Denaturation Heating leads to denaturation of the double-stranded DNA. 5 3 3 3 3 5 5 1. Start with a solution containing template DNA, synthesized primers, and an abundant supply of the four dNTPs. THE POLYMERASE CHAIN REACTION IS A WAY TO PRODUCE MANY IDENTICAL COPIES OF A SPECIFIC GENE
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Figure 19-7-2 5 4. Extension During incubation, Taq polymerase uses dNTPs to synthesize complementary DNA strand, starting at the primer. 5 3 3 3 3 5 5 3. Primer annealing At cooler temperatures, the primers bind to the template DNA by complementary base pairing.
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This note was uploaded on 01/21/2012 for the course BIOLOGY 172 taught by Professor Rajeshwarijanakiraman during the Spring '11 term at University of Michigan.

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Lecture 13 - 6:8 - Biology 172 Lecture 13: Wednesday, June...

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