Edman Degradation

Edman Degradation - H 5 H HO H Ring opening Ring closure...

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Mechanism of Edman Degradation for N-Terminal Analysis of Peptides C 6 H 5 N C S Phenyl isothiocyanate H 2 N C C R NH O Peptide NH C N C C S O H OH C 6 H 5 N C N C C S O H C 6 H 5 H R R H H S C C N C H H R NH O Peptide N C 6 H 5 S C C N C H H H R NH O Peptide N C 6 H 5 S C C N C H H R NH HO Peptide N C 6 H 5 H S C C N C H H R N O Peptide N C 6 H 5 H H H S C C N C H H R O N C 6 H 5 + H 2 O + H 3 O Thiazolinone Peptide-NH 2 S C C N C H H R O N C 6 H 5 H H 2 O C 6 H 5 N C S N CHR C O OH Proton shift S C C N C H H R O N C 6 H 5 H H 2 O HO S C C N C H H R O N C 6
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Unformatted text preview: H 5 H HO H Ring opening Ring closure formation of amide H H Phenylthiohydantoin derived from amino acid Proton shift same as NH C N C C S O H OH C 6 H 5 R H H...
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This note was uploaded on 01/21/2012 for the course ECON 2a taught by Professor Xi during the Spring '11 term at Cornell College.

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