Lecture+6-characterization+method+and+adult+stem+cell

Lecture+6-characterization+method+and+adult+stem+cell -...

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Unformatted text preview: Stem Cells and Therapies • How to identify and isolate stem cells • Adult stem cells How to Identify and Isolate Stem Cells/ Progenitor Cells • orphology Morphology • Markers: cell surface markers, transcriptional factors • Fluorescence-activated cell sorting (FACS) • Magnetic cell sorting • Differentiation potential Morphology Markers Oct-4, a transcription factor for embryonic stem cells Cell Surface Markers • Mouse embryonic stem cell (SSEA-1) • Hematopoietic stem cells (c-kit, CD34) • Bone marrow stromal cells (Stro-1) ( ) • Endothelial progenitors (Flk-1) A FACS Magnetic cell sorting (MACS) (1) Antibody-cell incubation (2) Add magnetic beads (~50 nm) conjugated with econdary antibody secondary antibody (3) Load the samples into a magnetic column (4) Wash (5) Elution after removing the magnet Differentiation Potential Bone marrow mesenchymal stem cells hondrogenic steogenic ndifferentiated Chondrogenic Osteogenic Undifferentiated CD105+, CD166+, CD29+, CD44+ CD34-, CD14-, CD45-...
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This note was uploaded on 01/21/2012 for the course BIOE 116 taught by Professor Various during the Fall '10 term at Berkeley.

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