Lecture+26-Gene+_+Protein+Delivery - DNA Delivery Delivery...

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Delivery Delivery of DNA of DNA and Proteins and Proteins DNA Delivery DNA Delivery Binding of DNA to cell membrane (mediated by surface charges) Internalization of DNA through cell membrane (usually through endocytosis) Trafficking of DNA through cytoplasm (need to survive endocytotic degradation) Entry of DNA into nucleus (through pores or during mitosis) Localization of DNA in nucleus (with or without incorporation into chromosomes) Methods of DNA Delivery (into nucleus of single cell) (~50% efficiency) (use electrical pulses to transiently permeabilize cell membranes) (Positive charged) Virus Adenovirus, retrovirus (high efficiency, but high toxicity)
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