This preview shows pages 1–3. Sign up to view the full content.
This preview has intentionally blurred sections. Sign up to view the full version.View Full Document
Unformatted text preview: Cryopreservation in tissue engineering 1. Tissue 1. Tissue Preservation Preservation Cryopreservation of cells Two-factor hypothesis Cryoprotectant additives Cryopreservation of tissue Barrier of transport process Other damage mechanisms Cryo Cryo-Effects Effects Preservation: Food, tissues and organs, ancient animals Damage: Cell lysis, cryo-surgery, frost damage to plants Tissue Preservation Tissue Preservation t t Room temperature: Easy to handle, short shelf life. Refrigeration Cryopreservation: Extended shelf life, more complications. Applications of Cryopreservation Technology Applications of Cryopreservation Technology in Tissue Engineering in Tissue Engineering creening source cells Screening source cells Cell banking Inventory control Quality control Biological manipulations Product distribution Tissue banking Challenges in Cryopreservation Protocol Development Challenges in Cryopreservation Protocol Development Cryopreservation causes significant changes in the thermal, chemical and physical environment, and thermal and chemical processing need to be optimized (composition, temperature, time) here are multiple steps and multiple factors There are multiple steps and multiple factors,...
View Full Document
- Fall '10
- Tissue Engineering