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Lecture+32-TissuePreserv-FDA

Lecture+32-TissuePreserv-FDA - • Cryopreservation in...

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Unformatted text preview: • Cryopreservation in tissue engineering 1. Tissue 1. Tissue Preservation Preservation • Cryopreservation of cells • Two-factor hypothesis • Cryoprotectant additives • Cryopreservation of tissue • Barrier of transport process • Other damage mechanisms Cryo Cryo-Effects Effects • Preservation: Food, tissues and organs, ancient animals • Damage: Cell lysis, cryo-surgery, frost damage to plants Tissue Preservation Tissue Preservation t t • Room temperature: Easy to handle, short shelf life. • Refrigeration • Cryopreservation: Extended shelf life, more complications. Applications of Cryopreservation Technology Applications of Cryopreservation Technology in Tissue Engineering in Tissue Engineering • creening source cells Screening source cells • Cell banking • Inventory control • Quality control • Biological manipulations • Product distribution • Tissue banking Challenges in Cryopreservation Protocol Development Challenges in Cryopreservation Protocol Development • Cryopreservation causes significant changes in the thermal, chemical and physical environment, and thermal and chemical processing need to be optimized (composition, temperature, time) here are multiple steps and multiple factors • There are multiple steps and multiple factors,...
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Lecture+32-TissuePreserv-FDA - • Cryopreservation in...

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