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Unformatted text preview: MSE/BioE 118 Lecture #15 Protein Adsorption and Cell Adhesion to Surfaces (Temenoff & Mikos Chapter 7.1 & 7.2, and Chapter 9) 1. Cell-Substrate Adhesion Overview 2. Matrix composition and specificity 3. Cell Surface • Receptors • Receptor-ligand engagement • Focal contact 4. Intracellular Events • Receptor clustering • Cytoskeleton • Focal Contacts 5. Measuring Cell-substrate adhesion 6. Design Example: Surface Modification of Polystyrene • Oxygen Plasma Treatment • Protein Adsorption and Cell Adhesion Protein Adsorption and Cell Adhesion to Surfaces 1. Cell-substrate adhesion Why is cell adhesion important? A number of biological processes depend on cell adhesion • Inflammation • Wound healing • Metastasis • Development • Regeneration of tissues and organs For biomaterials, biosensors, bioreactors, cell affinity chromatography • Attachment to the device • Fixation of the device • Population kinetics on the device • Overall biological performance The Cell-ECM Microenvironment Pol ock, J., and Healy, K.E., “Biomimetic and Bio-responsive Materials in Regenerative Medicine: Intel igent Materials for Healing Living Tissues,” In Strategies in Regenerative Medicine , M. Santin (Ed.), Springer, 2009 Matrix Proteins...
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- Fall '11
- Biomaterials, cell adhesion, cell-substrate adhesion