MSE-BioE+118+_11+L_24+TE_2

MSE-BioE+118+_11+L_24+TE_2 - MSE/BioE 118 Lecture #26...

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Unformatted text preview: MSE/BioE 118 Lecture #26 Tissue Engineering & Regenerative Medicine 1. Introduction to Tissue Engineering 2. Current Tissue Engineering Products 3. Cells in Tissue Engineering 4. Scaffolds in Tissue Engineering 5. Design Example: Artery Tissue Equivalent 6. Design Example: Tissue Engineered Bladder 7. Current Issues in Tissue Engineering 8. In Situ Regenerative Medicine - Bone Regeneration Dr. Anthony Atala Bladder reconstruction Bladder tissue biopsy Bladder cells are isolated and expanded Bladder shaped scaffold is constructed Scaffold is seeded with cells 3-D CT images are obtained for bladder scaffold design 6. Tissue Engineering of the Bladder Dr. Anthony Atala C Implantation of Engineered Bladder Oberpenning, F., et al., Nature Biotech. , 1999 Dr. Anthony Atala Implantation of Engineered Bladder Oberpenning, F., et al., Nature Biotech. , 1999 5 6 Hematoxylin and eosin histologic results (magnifcation X140) are shown For (A) normal canine bladder (group A), (B) the bladder dome oF the cellFree polymer reconstructed bladder (group B), and (C) the tissue engineered neoorgan (group C). Immunocytochemical staining oF tissueengineered neoorgan revealed (D) positive staining oF the epithelial cell layers with pancytokeratin AE1/ AE3 antibodies (original magnifcation X210); (E) urothelial diFFerentiationrelated membrane proteins, which constitute the apical plaques oF the asymmetric unit membrane (AUM) in normal urothelium (original magnifcation X210); () antialpha smooth muscle actin staining oF phenotypically normal smooth muscle (original magnifcation X210); and (G) positive staining with S100 antibodies binding to neural tissue and nerve sheaths (original magnifcation X320). Problems with Ex Situ Tissue Engineering Develop nerve instruction Develop vascular supply 7. Current Issues in Tissue Engineering Problems with Ex Situ Tissue Engineering Oxygen supply Nerve instruction Size or thickness of tissue equivalent limited Develop nerve instruction Develop vascular supply 7. Current Issues in Tissue Engineering Drug Delivery in Regenerative Medicine Develop vascular supply Develop nerve instruction 8. In Situ Regenerative Medicine Drug Delivery in Regenerative Medicine Develop vascular supply Develop nerve instruction 8. In Situ Regenerative Medicine Mechanical properties (e.g. G*, G, G) Drug Delivery in Regenerative Medicine Develop vascular supply Develop nerve instruction 8. In Situ Regenerative Medicine Mechanical properties (e.g. G*, G, G) Drug Delivery in Regenerative Medicine Develop vascular supply Develop nerve instruction 8. In Situ Regenerative Medicine Proteins Growth factor presentation Transcription Factors Antibodies Anti-Inflammatories Mechanical properties (e.g. G*, G, G) Drug Delivery in Regenerative Medicine Develop vascular supply Develop nerve instruction 8. In Situ Regenerative Medicine Proteins Growth factor presentation Transcription Factors Antibodies Anti-Inflammatories...
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This note was uploaded on 01/21/2012 for the course C 118 taught by Professor Hayleylam during the Fall '11 term at University of California, Berkeley.

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MSE-BioE+118+_11+L_24+TE_2 - MSE/BioE 118 Lecture #26...

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