MSE-BioE+2011+L_10-11+Protein+Ads

MSE-BioE+2011+L_10-11+Protein+Ads - MSE/BioE 118...

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MSE/BioE 118 Lectures #13-14 Protein Adsorption to Surfaces Outline 1. Overview of the Importance of Proteins in Biological Performance Protein adsorption Biological performance DLVO Theory Biological performance based on protein adsorption Cell response Why does adsorption and cell adhesion occur? 2. Physical and Chemical Adsorption 3. Protein Adsorption Experiment (virtual laboratory) 4. Competitive Protein Adsorption 5. Prime & Whitesides Paper 6. New Themes in Protein Adsorption 1. Overview of the Importance of Proteins in Biological Performance Protein adsorption Fields where protein adsorption is important Biomaterials Medical Devices Biotechnology (cell culture, protein processing, engineering) Biosensors/Diagnostics Biofouling (marine environments) Pharmaceuticals Filtration Nature 1. Overview of the Importance of Proteins in Biological Performance Biological Performance Host Response Initial Events Inflammation Immunological Enzymatic/biochemical Bacterial Neoplasia (cancer) Corrosion Fatigue Fracture Degradation Adsorption Absorption Resorption Material Response
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DLVO [Dejaguin, Landau (‘41); Verwey, Overbeek (48)] Theory Plate and a sphere (e.g., biomaterial and protein/cell/ bacteria) Initial events described by the colloid chemical approach Molecular adsorption- ions, small molecules Proteins, macromolecular adsorption Cell, bacteria, or particle adsorption Δ t = Vitronectin = Albumin Types of Forces Van der Waals Electrostatic Structural Interactions Short-range (acid-base) DLVO W(D) D(nm) * Need to understand van der Waals and electrostatic interactions to develop curves based on DLVO theory 1 o 2 o van der Waals attraction Electrostatic repulsion Importance of Proteins in Biological Performance W ( D ) = W E ( D ) + W A ( D ) + - - - - - - - - - + + + + + + + + + + + - - - - - - - - - - - - - - + + + + + + + + + + + - - - - - - - - - + + + + + + + + + - - - - - - - - Electrical double layer Electrostatic Forces Electrostatic interactions in a liquid medium Counter ion distribution Co-ion distribution ρ x = ion concentration or density = #/vol φ x = potential= V φ s = surface potential (x)
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DLVO [Dejaguin, Landau (‘41); Verwey, Overbeek (48)] Theory Plate and a sphere (e.g., biomaterial and protein/cell/bacteria) W(D) J or k T D(nm) 1 o 2 o van der Waals attraction
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