Unformatted text preview: ANAEROBIC GROWTH MEDIA AND METHODS Remember, strict or obligate anaerobes do not tolerate the presence of oxygen at normal atmospheric levels, although aerotolerant anaerobes can. Special media and techniques are required for strict anaerobes. 1. Special reducing media such as fluid thioglycolate medium can be used. Although this medium is slightly thickened with a small amount of agar, it basically is a liquid medium in tubes. Individual colonies cannot be seen. 2. If solid media are required, the plates are inoculated and quickly put into a special jar. Modern technique involves then activating a packet of chemicals and sealing the jar. The chemical reactions occurring in the sealed jar use up all the oxygen and leave anaerobic conditions. 3. In large labs, an anaerobic glove box chamber is used. This is a sealed transparent chamber which has all room air pumped out and replaced with inert gases after all microbes and necessary...
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- Fall '09