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DIRECT MEASUREMENT OF MICROBIAL GROWTH

DIRECT MEASUREMENT OF MICROBIAL GROWTH - . (ml)iscalculated...

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    DIRECT MEASUREMENT OF MICROBIAL GROWTH Liquid cultures are usually used and population numbers are recorded as the number of cells per  milliliter of the culture medium. Usually numbers in a very small sample are counted and then  the number per milliliter (ml) is calculated.  To get the small sample, a series of dilutions is performed. This is done because bacterial  numbers are usually so large that actually counting all the bacteria in 1 ml is difficult or  impossible. The original sample is usually diluted by taking 1 ml from it and putting it into  sterile liquid--either 1 ml into 9 ml (which gives a 1:10 dilution) or 1 ml into 99 ml (which gives  a 1:100 dilution). If necessary, the first dilution can be mixed and then 1 ml of this transferred to  9 or 99 ml again, and so on. When a "countable" dilution is achieved, the original number per ml 
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