GETTING THE DNA IN

GETTING THE DNA IN - GETTING THE DNA IN

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GETTING THE DNA IN   1. Transformation---bacteria will need to be treated to make them competent (receptive to taking  in the plasmid-donor gene complex). This is most often done as follows:      a. Bacteria that are to receive the plasmid are grown in a liquid medium.       b. Calcium chloride is added to the culture and the mixture is chilled.       c. Plasmid is added.      d. Cells are suddenly heated, which makes them receptive to taking in the plasmid.    2. Electroporation—an electrical current is used to make cells receptive.   3. Microinjection—DNA is injected through the plasma membrane using a micropipette.       IDENTIFYING CELLS WHICH HAVE TAKEN IN THE PLASMID
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This note was uploaded on 01/23/2012 for the course MCB MCB2010 taught by Professor Smith during the Fall '09 term at Broward College.

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GETTING THE DNA IN - GETTING THE DNA IN

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