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Unformatted text preview: If a count of the number of phage particles present is needed, solid media should be used. A pour plate is done by adding bacteria and a measured amount of a liquid containing phages to liquid agar and pouring it into a sterile Petri dish. Enough bacteria are included that without interference they would form a solid filmy growth across the entire surface of the agar (bacterial lawn). In every spot where a phage particle landed, the bacteria will be lysed and a small clear area called a plaque will appear. These can be counted. The number of viruses in the original viral suspension is also described as the number of plaque-formingunits....
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- Fall '09