GROWING BACTERIOPHAGES IN THE LABORATORY

GROWING BACTERIOPHAGES IN THE LABORATORY - If a count of...

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GROWING BACTERIOPHAGES IN THE LABORATORY Phages can be grown in liquid bacterial cultures or in bacterial cultures on solid media. The  bacteriophages were discovered by accident when a liquid culture of bacteria in a lab was  accidentally invaded by bacteriophages. Liquid cultures become quite cloudy as the numbers of  bacteria increase, but to the researcher’s surprise, a culture that was very cloudy and full of  bacterial growth one day was perfectly clear the next. If a little of this clear solution was added to  another cloudy culture, the same thing happened. Finally it was discovered that a virus was  attacking the bacteria and causing them to lyse (burst open). 
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Unformatted text preview: If a count of the number of phage particles present is needed, solid media should be used. A pour plate is done by adding bacteria and a measured amount of a liquid containing phages to liquid agar and pouring it into a sterile Petri dish. Enough bacteria are included that without interference they would form a solid filmy growth across the entire surface of the agar (bacterial lawn). In every spot where a phage particle landed, the bacteria will be lysed and a small clear area called a plaque will appear. These can be counted. The number of viruses in the original viral suspension is also described as the number of plaque-formingunits....
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