HOW DO WE GET THE GENE

HOW DO WE GET THE GENE - HOW DO WE GET THE GENE?

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HOW DO WE GET THE GENE? Many bacteria make enzymes that have the ability to cut a strand of DNA into pieces. These  enzymes are called restriction enzymes. A particular restriction enzyme always cuts into the  DNA at a particular sequence of bases. In the bacteria, these enzymes apparently are produced to  attack and destroy phage DNA. In the process, the cell attaches methyl groups to some of the  cytosine in its own DNA to protect it.       1. A restriction enzyme that cleaves the donor DNA in the proper place is located and mixed  with the donor DNA. This will result in the desired gene being cut away from the rest of the  chromosome.       2. The resulting DNA fragments are mixed with the plasmid and another enzyme, DNA ligase,  which can join DNA pieces together and splice the donor DNA into the plasmid. This forms a  recombinant DNA molecule.
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This note was uploaded on 01/23/2012 for the course MCB MCB2010 taught by Professor Smith during the Fall '09 term at Broward College.

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HOW DO WE GET THE GENE - HOW DO WE GET THE GENE?

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