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Unformatted text preview: LIGHT MICROSCOPY Modern microscopes are compound light microscopes. This means that there are 2 lenses that magnify, the ocular lens and the objective lens. Light rays provide the illumination. Brightfield microscopy--- this is the most common use of the light microscope. Specimens are usually stained to make them more easily visible. Light rays pass though the condenser lens and are directed straight up into the objective lens, passing through the specimen on the way. The field of view, seen through the ocular, is brightly illuminated, with the specimen appearing slightly darker if unstained, or brightly colored due to dyes. The total magnification provided can be determined by multiplying the ocular magnification times the objective magnification. The very best compound light microscope might have a top magnification near 2000X. Magnification alone is not the only concern--- a clear picture in which fine detail can be seen is...
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This note was uploaded on 01/23/2012 for the course MCB MCB2010 taught by Professor Smith during the Fall '09 term at Broward College.
- Fall '09