METHODS OF CLASSIFYING AND IDENTIFYING MICROORGANISMS RIBOSOMAL RNA SEQUENCING--t his is another technique used to determine the relationships among organisms. Ribosomal RNA is believed to change less than other nucleic acids over time, because if an organism has a drastic change in its rRNA it would be unable to assemble proteins and would die. A study of the similarity of rRNA of two different organisms is believed to show how far back in the past the two organisms branched off a common ancestor. POLYMERASE CHAIN REACTION--if even small amounts of the DNA of an organism can be obtained, a technique called the polymerase chain reaction can be used to increase the amount so that it can be tested. This technique is essential in studying causative agents of diseases which cannot be cultured in the laboratory. NUCLEIC ACID HYBRIDIZATION--to understand this technique, we must remember that
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