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Missouri Compromise - a.,.1mlofthedilutionis . .

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DIRECT MEASUREMENT OF MICROBIAL GROWTH a. POUR PLATES ---To make a pour plate, generally either 1 ml or 0.1 ml of the dilution is  placed in a sterile empty Petri dish. Nutrient agar which has been held in liquid form at about 45 C is added to the Petri dish and carefully mixed. The agar quickly solidifies and bacteria grow  spread out in the agar. Bacteria are exposed to the 45 o  C temperature for only a brief time. Most  species are not harmed by this brief exposure, but some of the most heat-sensitive types may be  damaged. Also colonies may not have their characteristic appearance when they grow below the  surface of the agar.  b. SPREAD PLATES -----A spread plate begins with a Petri dish with solid agar already in  place. .1 ml of a liquid sample is poured onto the surface of the agar and spread evenly over the  entire surface with a sterile implement. This method does not expose the microbes to heat and 
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