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Unformatted text preview: 4. Aerotolerant microbes do not use oxygen but can at least partly neutralize its toxic effects, so they can grow fairly well to very well in its presence. Energy is produced mainly by fermentation. 5. Microaerophiles must have oxygen but in very low levels. These require a little in at least some of their metabolic reactions but cannot tolerate normal atmospheric levels. Energy is produced by fermentation. The technique we use in lab to show these differing requirements is a special growth medium called fluid thioglycolate medium. This is placed in a deep test tube. It contains a very small concentration of agar to thicken it slightly, plus a chemical called sodium thioglycolate to tie up free oxygen. It is described as a reducing medium....
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- Fall '09