PREPARATION OF SPECIMENS FOR LIGHT MICROSCOPY

PREPARATION OF SPECIMENS FOR LIGHT MICROSCOPY -...

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PREPARATION OF SPECIMENS FOR LIGHT MICROSCOPY It is not impossible to view unstained microbes with bright-field microscopy.  This is usually  done by one of the following methods: T EMPORARY   WET   MOUNT ---a drop of liquid containing the microbes is placed on a slide.  A  cover slip is placed over the drop and the slide is viewed.  Lowering the condenser and  dimming the light a little may help.  Dabbing vaseline around the edges of the cover slip will  keep the specimen wet longer. HANGING   DROP ---a special slide with a hollowed out “well” in the center is used. A drop of  liquid containing the microbes is placed on a cover slip which has dabs of Vaseline on the  corners. The special slide is lowered over it, concave side down, until it touches the Vaseline.  The slide is then flipped over and viewed. .     
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PREPARATION OF SPECIMENS FOR LIGHT MICROSCOPY -...

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